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Expression levels of revTetR protein withing a prokaryotic host cell are varied and modulated by the choice of the promoter operatively associated with the structural gene encoding the revTetR protein.

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San Francisco Cable Car at Powell and Market Street

Gripmen in San Francisco changing direction at the turnable (Powell & Market St)
Gripmen in San Francisco changing direction at the turnable (Powell & Market St)

Ahh!! The world’s last manually operated cable car- a staple in the city of San Francisco. This dying breed had its genesis in 1873 after Andrew Smith Hallidie witnessed a terrible accident involving San Francisco’s notoriously steep hills, horse drawn carriages and too much weight.

Hallidie was a failed gold miner who cheated death on more than one occasion. He was reportedly caught in the midst of a forest fire, fell 25 feet from a suspension bridge, attacked by a band of Mexicans, lived through a blast in a shaft at the end of a 600 foot tunnel, and was the passenger in a runaway horse drawn stagecoach.  Yes he needed a change of luck!! But he was brilliant- he built his first wire suspension bridge at nineteen. But back to the trains!

After the horse tragedy in 1869, he immediately started the process to design and build the cable car.  The first cable car system was the Clay street line which made its debut in 1873 and cost $85,000 to build.  The bodies of these exquisite works of art are hand worked over the course of 18-24 months by carpenters using oak, Alaskan spruce covered in canvas, iron, steel and highly polished brass.  Its operators or Gripmen gained their unique name from the fact that at the “turnable” they literally have to grip the car and use their body weight to turn it around.  In 2010 they had their 2nd woman cable car operator and change the name to to grip person.

Unfortunately cable cars are becoming a rarity; San Francisco has less than 44 operational today a far cry from the 600+ at the turn of the 19th century.   The cars operate 7 days a week and you can indulge in Hallidie’s handiwork for $5.

The Venice Simplon Orient Express

The VSOE as it passes through Liechtenstein
The VSOE as it passes through Liechtenstein

 

The Venice Simplon-Orient-Express (VSOE) is the privately owned luxury rail liner that runs between London, Paris and Venice. It is famed as a descendent of the fabled Orient Express and each year it makes the long trek to Istanbul (Constantinople) following a similar path to that of its quasi parent launch in 1883. But book your tickets early because you can only ride this train from March through November. Its birth in 1982 came about as a result of a purchase of 1920’s and 30’s “Wagon- Lits” (sleep cars) by James Sherwood who reportedly spent $31M restoring the cars to their original opulence even down to the brass lamps and marquetry paneling

Luxury Trains|pictures of trains|trains around the world|

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